LncRNA RP11-551L14.4 suppresses breast cancer development by inhibiting the expression of miR-4472
LncRNA RP11-551L14.4 suppresses breast cancer development by inhibiting the expression of miR-4472
Blog Article
Background Previous studies have been reported that long non-coding RNA (lncRNA) can regulate the expression of genes which are involved in many important cellular processes The potential role of lncRNA RP11-551L14.4 in the development of breast cancer and the possible regulatory mechanisms was investigated.Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to analyze RP11-551L14.
4 expression in 36 paired breast cancer tissues and adjacent tissues.The expression of RP11-551L14.4 in multiple breast cancer cell lines was detected by qRT-PCR.
Meanwhile, overexpression of RP11-551L14.4 models was established using lentivirus in BT474 and T47D breast cancer cells.Cell counting kit-8 (CCK-8), cell colony formation and cell cycle assays were performed to detect the effects of RP11-551L14.
4 on the biological function of breast cancer cells.Besides, bioinformatics techniques, dual luciferase reporter gene assay and rescue 2.75x100 experiments were used to investigate the potential mechanisms.Results RP11-551L14.
4 expression was negatively associated with the advanced tumor stage.Breast cancer patients with low RP11-551L14.4 expression manifested a poorer prognosis.
The results of qRT-PCR showed that RP11-551L14.4 expression in breast cancer tissues was significantly lower than in adjacent tissues.Meanwhile, overexpression of RP11-551L14.
4 significantly decreased the cell proliferation and cell cycle.Bioinformatics technology showed that RP11-551L14.4 could complementarily bind to miR-4472.
qRT-PCR results indicated that the expression levels of miR-4472 and RP11-551L14.4 in breast cancer were negatively correlated.Luciferase reporter gene assay showed that miR-4472 remarkably decreased the relative luciferase activity of the wild-type RP11-551L14.
4 vector.miR-4472 is a direct target gene of RP11-551L14.4.
miR-4472 levels were reduced, and repulsive guidance molecule A (RGMA) mRNA or protein levels were increased dreamtops after overexpression of RP11-551L14.4 in the breast cancer cells.miR-4472 reversed the effects caused by RP11-551L14.
4 in breast cancer cells.Conclusion RP11-551L14.4 expression was remarkably decreased in breast cancer tissues and cells.
RP11-551L14.4 may inhibit the malignant progression of breast cancer by regulating miR-4472 expression.